SIBBM 2016 • Frontiers in Molecular Biology

SIBBM 2016
Frontiers in Molecular Biology

SIBBM 2016
Frontiers in
Molecular Biology

16-18 June, Naples

16-18 June

Società Italiana di Biofisica
e Biologia Molecolare
Società Italiana di Biofisica e Biologia Molecolare Società Italiana di Biofisica e Biologia Molecolare

Naples, 16-18 June 2016

From Single Cell Analysis to Precision Medicine


  • Thursday, 16 June

    12:30-14:30 Registration
    14:30-14:50 Welcome Addresses
      Gennaro Ciliberto, SIBBM president
    Luigi De Magistris, Mayor of Naples
    Gaetano Manfredi, Rector Univ. of Napoli Federico II and President CRUI
    Adolfo Gallipoli, President LILT Campania
    Session I Cell Heterogenity and Single Cell Analyses
    Chairs: Lucia Altucci and Tommaso Russo
    14:50-15:30 Stefano Gustincich, Genoa, Italy
    SINEUPs: a new functional class of antisense long non coding RNAs that activate translation
    15:30-15:50 Short talk: Valentina Proserpio, Hinxton, UK
    Single cell analysis of CD4+ T cell differentiation reveals three major cell states and progressive acceleration of proliferation
    15:50-16:30 Ido Amit, Rehovot, Israel
    Shaping the blood: lessons from chromatin and single cell RNA dynamics
    16:30-17:00 Coffee break
    17:00-17:40 TIGEM Lecture
    Sarah Teichmann, Cambridge, UK
    Understanding cellular heterogeneity
    17:40-18:20 Davide Cacchiarelli, Cambridge, MA, USA
    Investigate cell fate decisions through integrative genomics
    18:20-18:40 Short talk: Luisa Cutillo, Sheffield, UK
    Unsupervised clustering of cells and genes across conditions using scRNA-seq
    18:40-20:00 Poster Session I (odd numbers) & Wine and Cheese
  • Friday, 17 June

    Session II Functional Genomics and Genome Editing
    Chairs: Diego Di Bernardo and Caterina Missero
    8:30-9:10 Luigi Naldini, Milan, Italy
    Genetic engineering of hematopoiesis to treat inherited diseases and cancer
    9:10-9:30 Short Talk: Michela Serena, Verona, Italy
    CRISPR/Cas9 as a tool for studying the interactions between viral and cellular proteins
    9:30-9:50 Supporter talks
    Carlo Erba, Johanna De Castro Arce
    CRISPR-Cas9 genome engineering applications
    Promega, Giuseppe Mancuso
    Nanoluc®: welcome to the future of gene expression studies
    9:50-10:30 Epigen Lecture
    Denis Duboule, Lausanne, Switzerland
    Long range gene regulation during mammalian development
    10:30-11:00 Coffee break
    11:00-11:40 Jernej Ule, London, UK
    Trojan horses’ of splicing
    11:40-12:00 Short talk: Ivano Legnini, Rome, Italy
    Circular RNAs expression, function and protein-coding potential in myogenesis
    12:00-12:30 Supporter talks
    Merck Millipore, Carlo Raviolo
    Amnis: citofluorimetria e imaging insieme. Benefici rispetto alla citometria convenzionale
    M&M Biotech, Emma Nora Di Capua
    Advanced single cell isolation for Genomics
    Euroclone, Antonio Sorrentino
    Exosomal urinary miRNAs as promising biomarkers for prostate cancer
    12:30-13:10 Armenise special lecture
    Chair: Paolo Macchi
    Vincenzo Costanzo, Milan, Italy
    DNA damage response and DNA repair genes in human diseases
    13:10-14:00 Lunch
    Session III Molecular Basis of Cancer
    Chairs: Tiziana Bonaldi and Alfredo Budillon
    14:00-14:40 Fiona Watt, London, UK
    Understanding epidermal stem cell plasticity
    14:40-15:00 Short talk: Maria Chiara De Santis, Turin, Italy
    PI3KC2α, a new spindle associated protein involved in genomic instability and tumorigenesis
    15:00-15:40 Andrea Musacchio, Dortmund, Germany
    Feedback control of mitosis: learning from biochemical reconstitution
    15:40-16:00 Short talk: Luigi Fattore, Naples, Italy
    A complex network of miRNAs is responsible for the establishment of resistance to targeted therapies in metastatic melanoma
    16:00-16:40 Coffee Break & Poster Session II (even numbers) supported by Beckman Coulter
    16:40-17:20 Luciano Di Croce, Barcelona, Spain
    Gene regulation dynamics mediated by Polycomb complexes
    17:20-18:00 Bruno Amati, Milan, Italy
    Transcriptional programs and therapeutic targets in Myc-induced tumors
    Session IV Science & Society
    Chair: Valeria Poli
    18:00-18:30 Fiona Watt, London, UK
    Changing career landscape of scientists
    18:30-19:00 Roberto Di Lauro, Italian Embassy, London, UK
    Research and innovation: the UK model
    19:00-20:00 SIBBM Society meeting
    20:15 Social dinner
  • Saturday, 18 June

    Session V From Molecular Medicine to Clinics
    Marco Bianchi and Franco Salvatore
    8:30-9:10 Antonella De Matteis, Naples, Italy
    Mendelian disorders of membrane trafficking illuminate novel signalling pathways in the endomembrane system
    9:10-9:30 Short Talk: Maria Rosaria Mollo, Naples, Italy
    Mutant p63 leads to a TSLP-mediated autoimmune lymphoproliferative disorder in AEC syndrome
    9:30-10:10 Daniel S. Peeper, Amsterdam, Netherlands
    Large-scale genetic perturbations to reveal novel cancer vulnerabilities
    10:10-10:40 Coffee break
    10:40-11:20 Alberto Bardelli, Candiolo, TO, Italy
    Heterogeneity, drug resistance and clonal evolution of colorectal cancers
    11:20-11:40 Short Talk: Emanuela Teveroni, Rome, Italy
    Estrogens counteract differentiation impairment of FSHD myoblasts by antagonizing DUX4 activity
    11:40-12:20 René Bernards, Amsterdam, Netherlands
    Exploiting acquired vulnerabilities of drug resistant cancers
    12:20-13:00 Awards and concluding remarks
  • Posters

    Posters with an odd number are presented during Poster Session I (Thursday, 16 June).
    Posters with an even number are presented during Poster Session II (Friday, 17 June).

    The poster's maximum size is A0 (841 x 1189 mm; width x height).

    P.1 Ugo Ala (Turin)
    Into the ceRNA world: a new way of evaluating optimal cellular environments for competitive endogenous RNA cross-talk

    P.2 Susanna Ambrosio (Naples)
    LSD1 mediates MYCN control of epithelial-mesenchymal transition through silencing of metastatic suppressor NDRG1 gene

    P.3 Stefano Amente (Naples)
    High-resolution genome profiles of 8-oxodeoxyguanine, gH2AX and NBS1 reveal their co-association at transcribed long genes

    P.4 Tiziana Angrisano (Naples)
    Role of Ink4a/Arf in Precursor Pancreatic Cells homeostasis

    P.5 Luigi Aurisicchio (Rome)
    Targeting the Stroma to hit the tumor: MMP11 as a novel target for cancer immunotherapy

    P.6 Lidia Avalle (Turin)
    MicroRNAs-143 and -145 are induced by TGF-β and contribute to mammary epithelium EMT and tumour cell migration and invasion

    P.7 Monica Ballarino (Rome)
    Lnc-405 interacts with Purα/β proteins to regulate myogenic differentiation

    P.8 Laura Bavagnoli (Pavia)
    Characterization of miR-1307 by microarrays analysis

    P.9 Irene Bertolini (Milan)
    V-ATPase signaling in glioma

    P.10 Andrea Bianchi (Milan)
    Impairment of LaminA/C-Polycomb crosstalk as a possible epigenetic cause of Emery Dreifuss Muscular Dystrophy (EDMD)

    P.11 Stefano Biressi (Povo (TN))
    Muscle stem cell heterogeneity

    P.12 Francesca Ludovica Boffo (Naples)
    A molecular mechanism underlying the high frequency of silencing of tumour suppressor genes

    P.13 Rossana Bongianino (Pavia)
    Allele specific silencing of mutant mRNA rescues ultrastructural and arrhythmic phenotype in mice carriers of the R4496C mutation in the Ryanodine Receptor gene (RYR2)

    P.14 Viola Calabrò (Naples)
    The emerging relationship between Y box binding protein 1 and the cellular stress response

    P.15 Giorgia Careccia (Milan)
    Playing with HMGB1 in muscle: a master regulator of myogenesis?

    P.16 Davide Cassani (London, United Kingdom)
    Molecular Regulation of Actomyosin Cortex Mechanics

    P.17 Giuliana Catara (Naples)
    PARP1 activation regulates the PARP12 translocation from the Golgi complex to stress granules during oxidative stress

    P.18 Morena Catillo (Pavia)
    Splicing of transcripts for splicing factor SRSF1 is finely tuned in response to cell metabolism

    P.19 Nando Cervone (Naples)
    Structural requirements for Fcp1 phosphatase function in mitosis

    P.20 Chiara Ciardiello (Naples)
    Large oncosomes derived from the aggressive prostate cancer sub-line, DU145R80, can modify the biological behavior of the parental DU145 cells

    P.21 Bruna Codispoti (Catanzaro)
    Transient exposure to recombinant TAT-BMI-1 enhances the ex-vivo expansion of human cord blood-derived hematopoietic stem cells

    P.22 Antonino Colanzi (Naples)
    A JNK-GRASP65 signalling axis regulates ribbon formation trough the  acetylation of Golgi-originated tubulin

    P.23 Claudia Colussi (Rome)
    The nuclear pore protein Nup153 associates with chromatin and regulates cardiac gene expression in dystrophic mdx heart

    P.24 Lucia Coscujuela (Orbassano (TO))
    CircRNAs characterization in MCF7 breast cancer cells

    P.25 Susan Costantini (Naples)
    Human miR-544a interferes with expression of Selenoprotein K in hepatocarcinoma cell lines

    P.26 Sara Credendino (Naples)
    Characterization of a long non-coding RNA regulated in thyroid cancer

    P.27 Fabiola Curion (Pozzuoli (NA))
    Single cell RNA-sequencing of Rhodopsin depleted photoreceptors

    P.28 Cristina D'Aniello (Naples)
    Vitamin C and L-Proline antagonistic effects capture alternative states in the pluripotency continuum

    P.29 Maria Teresa De Angelis (Catanzaro)
    Short-term retinoic acid treatment maintains hPSCs in pluripotency state

    P.30 Nicola de Prisco (Pozzuoli (NA))
    Transcriptional blueprints of rods and cones

    P.31 Carmela Dell'aversana (Naples)
    miR-194-5p/BCLAF1 deregulation is a hallmark of AML tumorigenesis

    P.32 Rosa Della Monica (Naples)
    Antagonistic action of Cdk1 and Fcp1 on Arpp-19 phosphorylation controls mitotic progression

    P.33 Denise Drongitis (Naples)
    Preconditioning-dependent neuroprotection: epigenetic mechanism underlying this phenomenon in an in vitro cellular system

    P.34 Daniela Eletto (Salerno)
    Manipulation of the luminal domain of IRE1 determines UPR-mediated cell fate

    P.35 Carla Esposito (Naples)
    Selective combinatorial microRNA-based approach for glioblastoma cancer treatment 

    P.36 Roberta Esposito (Fisciano (SA))
    Role of the gastrointestinal TFF1 protein in the H. pylori infection

    P.37 Giulio Ferrero (Turin)
    Identification of critical enhancers by network modeling of long-range chromatin interactions

    P.38 Alessandro Fiorenzano (Naples)
    Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency

    P.39 Stefania Fochi (Verona)
    Human T-cell leukemia virus HBZ and APH-2 antisense proteins interaction with host factors and their involvement in NF-κB activation

    P.40 Rosa Fontana (Naples)
    Novel functions of the p14ARF tumor suppressor: deciphering cell fate at the crossroads of adhesion and survival

    P.41 Valentina Fustaino (Monterotondo (RM))
    Characterization of an EMT intermediate phenotype associated to resistance to EGFR inhibitors in non small cell lung cancer cell lines

    P.42 Miriam Gagliardi (Naples)
    ICF-specific DNMT3B mutations interfere with isoform-specific transcription

    P.43 Gennaro Gambardella (Naples)
    MicroRNAs shape the transcriptional landscape of ESCs by altering gene co-regulation within cells and transcriptional heterogeneity across cells

    P.44 Emilia Dora Giovannone (Catanzaro)
    Disseminated cancer cell in colon cancer identification, isolation and genetic characterization

    P.45 Giulia Guarguaglini (Rome)
    Aurora-A inhibition for anti-cancer therapies: canonical and innovative approaches

    P.46 Andrea Iannello (Orbassano (TO))
    Epigenetic control of Th17 and Treg cells balance upon E2 treatment

    P.47 Ambra Iannuzzi (Turin)
    Non canonical functions of STAT3 in regulating calcium signaling and resistance to apoptosis

    P.48 Eleonora Ingallina (Trieste)
    Identification of mutant p53 inhibitors by high-content screening

    P.49 Vincenzo Ingangi (Naples)
    The cyclic peptide [SRSRY] suppresses neovascularization and intravasation of chondrosarcoma cells

    P.50 Miroslava Kissova (Pavia)
    Identification of novel inhibitors of LRRK2 G2019S kinase mutant – a molecular determinant of Parkinson’s disease

    P.51 Patrizia Lavia (Rome)
    The GTPase RAN, nuclear transport receptors and kinetochore function during mitosis

    P.52 Francesca Lazzaroni (Milan)
    Leukemia on a chip: high content profiling at single-cell resolution

    P.53 Federica Liotti (Naples)
    IL-8 sustains an autocrine circuit necessary for thyroid cancer stem cells features

    P.54 Rita Lombardi & Maria Rita Milone (Mercogliano)
    Proteomic characterization of PPARg gamma knock in and knock out in two colorectal cancer cell models

    P.55 Debora Malpicci (Catanzaro)
    Dissecting early survival mechanisms in human melanoma exposed to targeted therapy

    P.56 Federica Marasca (Milan)
    Epigenetic role of DNA Transposable Elements (TEs) in shaping CD4+ T cell identity and plasticity

    P.57 Francesca Martinetto (Verona)
    Alternative splicing regulation of human FOXP2 speech gene

    P.58 Valentina Miano (Orbassano (TO))
    Estrogen receptor α-dependent, luminal subtype-specific long noncoding RNAs in breast cancer cells

    P.59 Maria Moretti (Pozzuoli (NA))
    Reverse-engineering the TFEB transcriptional network from time-course gene expression profiles reveals a specific timing of stress response components

    P.60 Angela Nebbioso (Naples)
    c-Myc modulation & acetylation is a key HDAC inhibitor target in cancer

    P.61 Viola Nervini (Povo (TN))
    Midbrain gene expression signatures of chronic cocaine abuse

    P.62 Alessia Noto (Rome)
    Stearoyl-CoA-Desaturase (SCD1) regulates lung cancer stemness via stabilization and nuclear localization of YAP/TAZ

    P.63 Gianmarco Pallavicini (Turin)
    Citron kinase as a therapeutic target for medulloblastoma

    P.64 Nadia Panera (Rome)
    LITAF silencing system to assess its tumour-suppressor role on hepatocarcinogenesis

    P.65 Emilia Pascale (Naples)
    Control of pluripotency and cell lineage determination mediated by a transcribed ultra-conserved non-coding RNA in embryonic stem cells

    P.66 Antonio Pezone (Naples)
    Epigenetic signatures in CDKN2A-B suppressor genes mark the evolution of myelodysplastic syndromes to acute myeloid leukemia in bone marrow cells with the same mutational spectrum

    P.67 Francesca Pezzuto (Naples)
    Mutation profile of TERT promoter and beta-catenin gene in hepatitis B and hepatitis C related hepatocellular carcinoma

    P.68 Giulia Piaggio (Rome)
    Mito-Luc Zebrafish model to visualize proliferation events in whole animals

    P.69 Maria elena Pisanu (Rome)
    Stearoyl-CoA-Desaturase 1 (SCD1) regulates lung cancer stem cells survival and is a prognostic factor  in lung adenocarcinoma

    P.70 Loredana Poeta (Naples)
    A “fault disease model” found in neurodevelopmental disorders (NDDs) is induced by a damage in the regulatory axis ARX/KDM5C/H3k4me3

    P.71 Dario Presutti (Monterotondo (RM))
    MET gene amplification and MET receptor activation are not sufficient to predict efficacy of combined MET and EGFR inhibitors in EGFR TKI-resistant NSCLC cells

    P.72 Nella Prevete (Naples)
    Formyl Peptide Receptor 1 exploits ω-3/ω-6 fatty acid-derived pro-resolving mediators to hamper gastric cancer angiogenesis and progression

    P.73 Salvatore Pulcrano (Naples)
    Identification of novel molecular players during the differentiation of dopaminergic neurons

    P.74 Bernd Pulverer (Heidelberg, Germany)
    Transparent Publishing - how to share reproducible data

    P.75 Erika Giorgia Quaranta (Padua)
    YAP/TAZ/TEAD cooperate with AP-1 for growth control and tumorigenesis

    P.76 Lorena Quirico (Turin)
    miR-148b and miR-214 as putative therapeutic targets

    P.77 Ilaria Romito (Rome)
    FAK knockdown reduces in vitro and in vivo hepatocarcinoma growth

    P.78 Lorenza Ronfani (Milan)
    Generation of genetically modified mice using the CRISPR-Cas9 technology

    P.79 Aniello Russo (Caserta)
    MicroRNA-125a-5p is a downstream effector of sorafenib in its antiproliferative activity toward human hepatocellular carcinoma cells

    P.80 Claudia Russo (Naples)
    Mutations in the carboxyl terminal domain of p63 causative of AEC syndrome lead to protein aggregation and reduced DNA binding

    P.81 Giusi Russo (Naples)
    DNA damage and Repair Modify DNA and Chromatin. Mechanism of allele methylation polymorphism

    P.82 Marco Santorelli (Sarno)
    Reconstitution in mammalian cells of the Hes1 oscillator by a synthetic biology approach

    P.83 Giovanni Saraceno (Trento)
    Predictive biomarker of response to seasonal influenza vaccination

    P.84 Giusy Sergi (Povo (TN))
    Biomarker of kidney rejection based on blood transcriptional profiles

    P.85 Angela Serpico (Naples)
    Fcp1 phosphatase controls RepoMan-PP1 complex formation for timely completion of mitosis

    P.86 Giacomo Siano (Pisa)
    ​Imaging the impact of Tau three-dimensional conformation on microtubules interplay and aggregation

    P.87 Melissa Sorci (Rome)
    Unraveling the function of m6A modification in acute myeloid leukemia

    P.88 Alessandro Testori (Naples)
    Shared genetic susceptibility among neural crest cell-derived cancers

    P.89 Donatella Vairo (Brescia)
    In vitro assays for discrimination between monogenic and acquired systemic interferonopathies by signal transducers assessment

    P.90 Elena Valentino (Trieste)
    Mutant p53 sustains a pro-oncogenic response to insulin in hormone-independent breast and prostate cancer

    P.91 Alessandra Vollero (Pavia)
    Efficacy of a biological therapy for recessive Catecholaminergic Polymorphic Ventricular Tachycardia in human induced pluripotent stem cells-derived cardiomyocytes

    P.92 Samuel Zambrano (Milan)
    Synchronous NF-κB oscillations translate into functionally related patterns of gene expression